BAMS™: Bead Assisted Mass Spectrometry

A new tool for targeted, multiplexed proteomics that combines bead-based affinity enrichment with the power of mass spectrometry

HIGH DENSITY

thousands of protein targets can be monitored in a single assay

HIGH CONTENT

protein identification and quantification using mass spectrometry for thousands of assays in a single run

ECONOMICAL

microarray format provides low cost per assay

SENSITIVE

low femto-mole (or low pg/mL) detection limit

QUANTITATIVE

established MALDI MS platform provides 3-4 orders of magnitude dynamic range

REPRODUCIBLE

variation is typically less than 15% CV within analytical replicates

FLEXIBLE

can be customized to incorporate additional protein targets of interest

SELECTIVE

immuno-affinity capture provides 1000-10000 level fold enrichment of target protein analytes

BAMS™ assay workflow:

Sample Type

tissue, cell line cultures, liquid biopsies

Generate Lysate

proteins are extracted from original source to make accessible for BAMS™ assay

Digest Protein Lysate

proteins are digested with protease (e.g. trypsin or chymotrypsin) for immuno-affinity capture

Affinity Capture

BAMS™ affinity capture beads are used to enrich for corresponding peptides to each target protein or post-translational modification (PTM)

Prepare BAMS™ Array

BAMS™ affinity capture beads are washed and then assembled into an ordered array using Adeptrix’ pico-well chamber gasket

Scan BAMS™ Array

captured peptides are eluted from beads and deposited onto microarray slide for MALDI MS scanning

Identify Protein Targets

acquired MALDI MS signal (mass & intensity) is compared to spectral library for identification of target protein or PTM

Quantify Protein Targets

MALDI MS intensity signal is used to determine relative quantitation based on internal standard reference

Monitor Biological Pathways

BAMS™ assay can be assembled with a specific panel of proteins and PTMs to monitor key signaling networks or specific areas of biology